Use of Kl LAC4 as Reporter
Gene in Candida albicans
CHEN Xi, CHEN Jiang-Ye*
( State Key Laboratory of Molecular Biology, Shanghai Institute of
Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China
)
Abstract The lacZ
gene which codes for ¦Â-galactosidase from E.coli does not work in Candida
albicans. In this work a reporter system with Kl LAC4
gene was contructed, coding for ¦Â-galactosidase from Kluyveromyces lactis.
Kl LAC4 gene was fused to the promoter of ADH1 of Candida
albicans with the terminator sequence of ADH1 at the tail. The
constructed plasmid,named pYPB1-LAC4, was used to transform Candida
albicans. ¦Â-galactosidase activity was measured by plate assay, when
cultured on solid medium, and by photometric assay, when cultured in liquid
medium. The results suggest that LAC4 of Kluyveromyces lactis
can serve as a reporter gene in Candida albicans.
Key words Candida albicans£» reporter gene£» Kl LAC4£» ¦Â-galactosidase
*Corresponding author£º Tel, 86-21-64374430-251£» Fax, 86-21-64338357£» e-mail, [email protected]